Tannin-Tolerant and Extracellular Tannase Producing Bacillus Isolated from Traditional Fermented Tea Leaves and Their Probiotic Functional Properties

摘要: A total of 117 Bacillus strains were isolated from Miang, a culture relevant fermented tea northern Thailand. These collected 16 sampling sites in north In this collection 95 isolates tannin-tolerant capable growth on nutrient agar supplemented with 0.5% (w/v) tannins leaves extract (TE). The also positive for pectinase, xylanase and amylase activity, while 91 86 cellulase β-mannanase, respectively. Only 21 producing extracellular tannase selected further characterization. Identification by 16S rRNA gene sequence analysis revealed that more than 50% (11 isolates) tequilensis, whereas the remaining B. siamensis (3), megaterium aryabhattai (3) toyonensis (1). tequilensis K34.2 produced highest activity 0.60 U/mL after cultivation at 37 °C 48 h. addition, all resistant to 0.3% bile salt, sensitive gentamicin, erythromycin, vancomycin kanamycin tolerant acidic condition. Cell hydrophobicity varied 9.4 80.4% neutralized supernatants some showed bacteriocin potentiality against Samonella enterica serovar Typhimurium TISTR 292. All tested probiotic properties indicated K19.3, K19.1 had high potential. This is first report describing their capability traditional