Angiogenic Gene-Modified Muscle Cells for Enhancement of Tissue Formation
作者： Paolo De Coppi , Dawn Delo , Lynn Farrugia , Kavid Udompanyanan , James J. Yoo
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摘要: Engineering muscle tissue with inadequate vascularity may lead to fibrosis and loss of function. In this study we combined the isolation genetic engineering myoblasts transplantation in an attempt create well-vascularized tissue. Myoblasts were obtained from a single explant adult Lewis rat myofibers transfected bicistronic plasmid encoding vascular endothelial growth factor (VEGF) green fluorescent protein (GFP) or nonfunctional VEGF–alkaline phosphatase (AP) fusion protein. VEGF expression GFP vitro were, respectively, assessed by Western blot analysis ELISA fluorescence microscopy, showing that successfully expressing recombinant proteins. The cells suspended collagen type I injected subcutaneously into nude mice. Analysis retrieved engineered tissues RT-PCR immunostaining showed Immuno...
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