Cellular concentrations of protein kinase A modulate prostaglandin and cAMP induction of alkaline phosphatase.

摘要: The relationship between the concentration of cAMP-dependent protein kinase (PKA) activity and induction alkaline phosphatase (AP) was examined in transfected L cell lines with altered PKA levels. C alpha 12 cells were generated by transfecting mouse an expression vector coding for catalytic subunit shown to contain 2.5-fold more than cells. RAB10 transfection a mutant regulatory had 10-fold lower levels AP 8-chlorophenylthio-cAMP (CPT-cAMP) found be 2-fold greater cells, while lacked any response CPT-cAMP. Northern blot solution hybridization analyses mRNA showed that induced comparable Western analysis demonstrated suggested increased level resulted from either stability or rate translation mRNA. In contrast, failed detect after CPT-cAMP treatment is required Stimulation endogenous cAMP prostaglandin E1 gave similar effects on as those seen These results indicate cellular can determine magnitude hormonal stimulation also suggest regulate gene at both protein.