Subcritical water extraction of polyphenolic compounds from sorghum (Sorghum bicolor L.) bran and their biological activities.

摘要: Abstract Subcritical water extraction (SWE), an environment-friendly technique, was applied to extract polyphenolics from sorghum bran. Extraction temperatures (°C), time (min), and solid-liquid ratio (mL/g) were investigated optimized by Box-Behnken design. The conditions for SWE 144.5 °C of temperature, 21 min time, 35 mL/g ratio, with a yield 47.253 ± 0.375 mg GAE/g dw, which in good agree the predicted value. Comparing hot (HWE), resulted higher polyphenolics, radical scavenging activities, more efficient antiproliferative activity. Furthermore, major polyphenolic compositions extracts identified quantified HPLC-ESI-MS/MS. Taxifolin, taxifolin hexoside, oligomeric procyanidins, epicatechin most abundant compounds extracts. Taken together, can be used as effective method bran, could potential source natural antioxidants.