REDUCTION AND ALKYLATION OF HUMAN PLASMA OROSOMUCOID IN AQUEOUS SOLUTION AND IN 8 M UREA: A PHYSICOCHEMICAL AND IMMUNOCHEMICAL STUDY: HUMAN OROSOMUCOID: REDUCTION AND ALKYLATION
作者： W. A. Krotoski , H. E. Weimer
DOI: 10.1139/O67-187
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摘要: Highly purified orosomucoid (α1-acid glycoprotein) was exposed to mercaptoethanol, iodoacetamide and 8 M urea, in various combinations. The derivatives were examined by agar-gel electrophoresis, by...
nrcresearchpress.com参考文章(28)
Hans Neurath, The proteins : composition, structure, and function ,(2012)
R. D. Lillie, Histopathologic Technic and Practical Histochemistry ,(1954)
J. Leggett Bailey, Techniques in protein chemistry Elsevier Pub. Co.. ,(1967)
Milton. Winitz, Jesse P. Greenstein, Chemistry of the amino acids Wiley. ,(1961)
William E. Marshall, The Structure of Glycoproteins II. THE SEPARATION AND CHARACTERIZATION OF THE POLYMORPHIC VARIANTS OF THE α1-ACID GLYCOPROTEIN OF HUMAN SERUM Journal of Biological Chemistry. ,vol. 241, pp. 4731- 4737 ,(1966) , 10.1016/S0021-9258(18)99707-3
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
N. M. Alexander, Spectrophotometric Assay for Sulfhydryl Groups Using N-Ethylmaleimide Analytical Chemistry. ,vol. 30, pp. 1292- 1294 ,(1958) , 10.1021/AC60139A031
Je H. Kim, Basudev Shome, Ta-Hsiu Liao, John G. Pierce, Analysis of neutral sugars by gas-liquid chromatography of alditol acetates: Application to thyrotropic hormone and other glycoproteins Analytical Biochemistry. ,vol. 20, pp. 258- 274 ,(1967) , 10.1016/0003-2697(67)90031-0
W.A. Krotoski, H.E. Weimer, Peptide-associated and antigenic changes accompanying periodic acid oxidation of human plasma orosomucoid Archives of Biochemistry and Biophysics. ,vol. 115, pp. 337- 344 ,(1966) , 10.1016/0003-9861(66)90284-0
W. A. Krotoski, D. C. Benjamin, H. E. Weimer, EFFECTS OF STARCH CONCENTRATION ON THE RESOLUTION OF SERUM PROTEINS BY GEL ELECTROPHORESIS: A SIX-SPECIES COMPARISON Biochemistry and Cell Biology. ,vol. 44, pp. 545- 555 ,(1966) , 10.1139/O66-066