Evaluation of Pre-analytical Variables in a Commercial Thrombin Generation Assay

摘要: Abstract Background There is minimal data on the influence of pre-analytical variables use calibrated automated thrombography (CAT), to measure thrombin generation. Objectives To evaluate impact centrifugation methods, time after collection, and contact activation inhibition CAT assay performed using two commercial reagents. Methods Results Six different methods plasma separation were examined. Thrombin generation triggered by a 5 pM tissue factor reagent was not greatly affected method, with similar results obtained all apart from single membrane filtration. Membrane filtration increased APTT recommended. Extended double at higher speed required minimise residual phospholipid 1 trigger, particularly activation. The effect delay up 24 hours in preparing assessed. No significant difference observed among samples processed between 0.5 6 hours blood collection into plastic Vacuette® tubes. presence or absence corn trypsin inhibitor had parameters minor differences seen Peak ttPeak factor. Conclusions dependent concentration trigger used. are sensitive method activation, standardisation allow large collaborative studies be performed.