Nontoxic Genetic Engineering of Mesenchymal Stem Cells Using Serum-Compatible Pullulan-Spermine/DNA Anioplexes
作者： Devang K. Thakor , Yang D. Teng , Hideaki Obata , Kentaro Nagane , Shigeru Saito
DOI: 10.1089/TEN.TEC.2010.0120
关键词:
摘要: Genetic modification of stem cells could be applied to initiate/enhance their secretion therapeutic molecules, alter biological properties, or label them for in vivo tracking. We recently developed a negatively charged gene carrier (“anioplex”) based on pullulan-spermine, conjugate prepared from natural polysaccharide and polyamine. In rat mesenchymal (MSCs), anioplex-derived reporter activity was comparable exceeded that obtained using commercial cationic lipid reagent. Transfection the growth medium with 15% serum antibiotics approximately sevenfold more effective than serum-free conditions. Cytotoxicity essentially indiscernible after 24 h anioplex transfection 20 μg/mL DNA, contrast resulted 40%–60% death target MSCs. Anioplex-derived persisted throughout entire 3-week study, post-transfection MSCs appearing maintain osteogenic, adipogenic, chondrogenic mul...
liebertonline.com参考文章(56)
Sujatha Dokka, David Toledo, Xianglin Shi, Vincent Castranova, Yon Rojanasakul, Oxygen radical-mediated pulmonary toxicity induced by some cationic liposomes. Pharmaceutical Research. ,vol. 17, pp. 521- 525 ,(2000) , 10.1023/A:1007504613351
Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton, Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells. ,vol. 25, pp. 2739- 2749 ,(2007) , 10.1634/STEMCELLS.2007-0197
Karin Corsi, Fatiha Chellat, Julio C Fernandes, None, Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. Biomaterials. ,vol. 24, pp. 1255- 1264 ,(2003) , 10.1016/S0142-9612(02)00507-0
Jun-ichiro Jo, Tomonori Ikai, Arimichi Okazaki, Masaya Yamamoto, Yoshiaki Hirano, Yasuhiko Tabata, Expression profile of plasmid DNA by spermine derivatives of pullulan with different extents of spermine introduced Journal of Controlled Release. ,vol. 118, pp. 389- 398 ,(2007) , 10.1016/J.JCONREL.2007.01.005
Victor Vacanti, Elton Kong, Gen Suzuki, Kazuki Sato, John M. Canty, Techung Lee, Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture Journal of Cellular Physiology. ,vol. 205, pp. 194- 201 ,(2005) , 10.1002/JCP.20376
Edmund R. Hollis, Paul Lu, Armin Blesch, Mark H. Tuszynski, IGF-I gene delivery promotes corticospinal neuronal survival but not regeneration after adult CNS injury. Experimental Neurology. ,vol. 215, pp. 53- 59 ,(2009) , 10.1016/J.EXPNEUROL.2008.09.014
Erin D. Milligan, Evan M. Sloane, Stephen J. Langer, Travis S. Hughes, Brian M. Jekich, Matthew G. Frank, John H. Mahoney, Lindsay H. Levkoff, Steven F. Maier, Pedro E. Cruz, Terence R. Flotte, Kirk W. Johnson, Melissa M. Mahoney, Raymond A. Chavez, Leslie A. Leinwand, Linda R. Watkins, Repeated intrathecal injections of plasmid DNA encoding interleukin-10 produce prolonged reversal of neuropathic pain. Pain. ,vol. 126, pp. 294- 308 ,(2006) , 10.1016/J.PAIN.2006.07.009
Darwin J Prockop, Repair of tissues by adult stem/progenitor cells (MSCs): controversies, myths, and changing paradigms. Molecular Therapy. ,vol. 17, pp. 939- 946 ,(2009) , 10.1038/MT.2009.62
Tetsuro Tanaka, Shinya Hamano, Yuko Fujishima, Yoshiharu Kaneo, Uptake of Pullulan in Cultured Rat Liver Parenchymal Cells Biological & Pharmaceutical Bulletin. ,vol. 28, pp. 560- 562 ,(2005) , 10.1248/BPB.28.560
Scott M. Vickers, Tobias Gotterbarm, Myron Spector, Cross-linking affects cellular condensation and chondrogenesis in type II collagen-GAG scaffolds seeded with bone marrow-derived mesenchymal stem cells Journal of Orthopaedic Research. ,vol. 28, pp. 1184- 1192 ,(2010) , 10.1002/JOR.21113