GCˆGC-TOFMS for the Analysis of Metabolites Produced by Termites (Reticulitermes flavipes) Bred on Different Carbon Sources

摘要: More and more studies are dedicated to termites their symbionts, better understand how they efficiently produce energy from lignocellulose. In that context, a powerful analytical method was developed perform the detection, separation identification of compounds in 1 µL fluid volume gut termite Reticulitermes flavipes. Comprehensive two-dimensional gas chromatography (GC×GC) coupled time-of-flight mass spectrometry (TOFMS) tested with three different column combinations: (1) low-polar/mid-polar; (2) polar/low-polar (3) mid-polar/low-polar. The set offered best chosen for further analysis comparison study. Metabolites were detected samples, including amino acids, sugars, amines organic acids. Samples collected fed 30 days on Avicel cellulose or xylan powder diets analyzed compared wood diet. Principal component (PCA) metabolite profiles demonstrated clusters corresponding diets, similar trend containing cellulose. Analysis variance (ANOVA) (one way-ANOVA Tukey’s test) used compare compound levels between these diets. Significant differences observed, higher aromatic derivatives diet sugar alcohols A accumulation uric acid observed artificial (cellulose xylan), likely be related nitrogen deficiency. present study highlighted capability adaptation system non-optimal carbon sources subsequent modification profile. These results demonstrate potential interest investigate profiling state-of-the-art science tools, order extract information could integrated other omics data provide insight into termite-symbiont digestion system.