RNA folding dynamics by single-molecule fluorescence resonance energy transfer
作者： Rui Zhao , David Rueda
DOI: 10.1016/J.YMETH.2009.04.017
关键词:
摘要: Single-molecule fluorescence resonance energy transfer (smFRET) microscopy has become an increasingly popular tool to study the structural dynamics of RNA molecules. It reveals, in real time, these molecules that would be otherwise hidden ensemble-averaged measurements. Here we present a detailed protocol for performing smFRET using total internal reflection microscopy, including preparation, optical setup, separation dual color channels, sample immobilization and data acquisition analysis.
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