作者: X.L. ZHANG , Y.Q. LI , S.H. CHEN , L.J. YANG , S. CHEN
DOI: 10.1111/J.1751-553X.2008.01091.X
关键词:
摘要: … After 3-min denaturation at 94 C, 40 PCR cycles were performed, each cycle consisting of 94 C for 1 min, 60 C for 1 min and 72 C for 1 min, and a final 7-min elongation at 72 C. Then, …