Analysis of leucine enkephalin by high-performance liquid chromatography using enzymatic derivatization by tyrosinase and electrochemical or fluorescence detection.
作者： Véronique Larsimont , Marja Bogaards , Pascalle Mulleneers , Günther Hochhaus
DOI: 10.1016/S0731-7085(98)00141-1
关键词:
摘要: Two tyrosine specific, HPLC methods with either electrochemical (HPLC-ED) or fluorescence (HPLC-FL) detection are described for leucine-enkephalin (LE) in cerebrospinal fluid (CSF). Both approaches involve the hydroxylation of Tyr1-moiety LE by mushroom tyrosinase. Production a catechol permitted selective clean-up analyte using boronate gels and produced species which were more readily oxidizable than LE. The controlled oxidation to corresponding chinones enabled reaction 1,2-diamino-1,2-diphenylethane (DPE) subsequent quantification HPLC-FL. HPLC-ED HPLC-FL yielded limits CSF 360 500 fmol per injection, respectively. Inter-day variability calibration curve samples was lower 20% slightly higher assay.
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sci-hub.se 参考文章(19)
Rao S. Rapaka, Frank Porreca, Development of delta opioid peptides as nonaddicting analgesics. Pharmaceutical Research. ,vol. 8, pp. 1- 8 ,(1991) , 10.1023/A:1015809702296
Guo-Qing ZHANG, Masaaki KAI, Mutsuko NAKANO, Yosuke OHKURA, Pre-column fluorescence derivatization high-performance liquid chromatography of opioid peptides in rat brain and its use for enzymatic peptide characterization. Chemical & Pharmaceutical Bulletin. ,vol. 39, pp. 126- 129 ,(1991) , 10.1248/CPB.39.126
Mutsuko Nakano, Masaaki Kai, Masahiro Ohno, Yosuke Ohkura, High-performance liquid chromatography of N-terminal tyrosine-containing oligopeptides by pre-column fluorescence derivatization with hydroxylamine, cobalt (II) and borate reagents. Journal of Chromatography A. ,vol. 411, pp. 305- 311 ,(1987) , 10.1016/S0021-9673(00)93981-5
Junichi Ishida, Masaaki Kai, Yosuke Ohkura, High-performance liquid chromatography of tyrosine-containing peptides by pre-column derivatization involving formylation followed by fluorescence reaction with 1,2-diamino-4,5-dimethoxybenzene. Journal of Chromatography A. ,vol. 356, pp. 171- 177 ,(1986) , 10.1016/S0021-9673(00)91476-6
KJ Dave, JF Stobaugh, TM Rossi, CM Riley, None, Reversed-phase liquid chromatography of the opioid peptides. 3. Development of a microanalytical system for opioid peptides involving microbore liquid chromatography, post-column derivatization and laser-induced fluorescence detection Journal of Pharmaceutical and Biomedical Analysis. ,vol. 10, pp. 965- 977 ,(1992) , 10.1016/0731-7085(91)80106-J
P De Montigny, CM Riley, LA Sternson, JF Stobaugh, None, Fluorogenic derivatization of peptides with naphthalene-2,3-dicarboxaldehyde/cyanide: optimization of yield and application in the determination of leucine-enkephalin spiked human plasma samples. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 8, pp. 419- 429 ,(1990) , 10.1016/0731-7085(90)80070-6
Hitoshi Nohta, Akane Mitsui, Yosuke Ohkura, Spectrofluorimetric determination of catecholamines with 1,2-diphenylethylenediamine Analytica Chimica Acta. ,vol. 165, pp. 171- 176 ,(1984) , 10.1016/S0003-2670(00)85197-1
M. Tellier, R.J. Prankerd, G. Hochhaus, A new fluorogenic assay for tyrosine-containing peptides. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 9, pp. 557- 563 ,(1991) , 10.1016/0731-7085(91)80177-B
Stefan Müller, Bert Ho, Petro Gambus, William Millard, Günther Hochhaus, An HPLC/RIA method for dynorphin A1-13 and its main metabolites in human blood Journal of Pharmaceutical and Biomedical Analysis. ,vol. 16, pp. 101- 109 ,(1997) , 10.1016/S0731-7085(97)00010-1
C.M.B. van den Beld, U.R. Tjaden, N.J. Reinhoud, D.S. Stegehuis, J. van der Greef, Determination of some β-endorphin fragments in human plasma by high-performance liquid chromatography with laser induced fluorescence detection☆ Journal of Controlled Release. ,vol. 13, pp. 129- 139 ,(1990) , 10.1016/0168-3659(90)90004-D