Modulation of single Ca2+-dependent K+-channel activity by protein phosphorylation.

摘要: There is considerable evidence that cyclic AMP can modulate the electrical activity of excitable cells1–10 and protein phosphorylation by catalytic subunit (CS) cAMP-dependent kinase a necessary step in these modulatory effects11–21. In analogy to alterations enzyme activities following phosphorylation12, it seems possible direct ion-channel proteins may alter their gating properties, giving rise observed changes activity. However, results obtained so far do not indicate whether ion channels themselves are phosphorylated, or simply an early some cascade events which leads ultimately modulation channel The development single-channel recording techniques22 has provided way investigate this question. Here we describe effects CS on individual Ca2+-dependent K+ from nervous system land snail Helix measured isolated membrane patches artificial phospholipid bilayers. demonstrate produces long-lasting channels, relevant site closely associated with channel.