Simultaneous Measur of Cell (Ca) and glr Explore Calcium's Role

作者： Marc C. Debell , Shirley M. E. Wong , Abeba Tesfaye

DOI:

关键词:

摘要: To explore the role of calcium in mediating action carbachol chloride-secreting epithelia, we simultaneously measured intracellular free (Ca) ((Ca).) and potassium conductance (gx) basolateral membrane T84 cells grown on collagen-coated filters. (Call was with fura-2 fluores- cence microscopy expressed as a relative value ((Ca)() normalized to control. assess changes gK, short circuit current (I~ presence luminal amphotericin transepithelial mucosa-to-serosa K + gradient (Germann, W.J., M. E. Lowy, S. A. Ernst, D. C. Dawson. 1986.J. Gen. Physiol. 88:237-251). Treatment monolayers resulted parallel increase then decrease (Ca)~ gx. The carbachol-induced gg appeared be dependent (Ca)i because stimulation ofg x significantly diminished when hormone-induced blunted, either by loading BAPTA or reducing extracellular (Ca). carbachol-stimulated gK direct result steady-state (Ca)~. (C,a)~ after were correlated ionomycin also increased manner. Agi~ per A(Ca)~, however, greater than that ionomycin. Because appear open same channel, conclusion based inhibitor selectivity experiments, may have second amplifies effect

暂无可下载资源，当前可以选择系统获取到有开放资源时通知我或者直接发起求助文献求助

参考文章(32)

Ole H. Petersen, Yoshio Maruyama, Calcium-activated potassium channels and their role in secretion Nature. ,vol. 307, pp. 693- 696 ,(1984) , 10.1038/307693A0

Reinhold Penner, Gary Matthews, Erwin Neher, Regulation of calcium influx by second messengers in rat mast cells Nature. ,vol. 334, pp. 499- 504 ,(1988) , 10.1038/334499A0

Ramon Latorre, Christopher Miller, Conduction and selectivity in potassium channels. The Journal of Membrane Biology. ,vol. 71, pp. 11- 30 ,(1983) , 10.1007/BF01870671

Douglas A. Ewald, Alan Williams, Irwin B. Levitan, Modulation of single Ca2+-dependent K+-channel activity by protein phosphorylation. Nature. ,vol. 315, pp. 503- 506 ,(1985) , 10.1038/315503A0

R. Hinrichsen, A Burgess-Cassler, B. Soltvedt, T Hennessey, C Kung, Restoration by calmodulin of a Ca2+-dependent K+ current missing in a mutant of Paramecium. Science. ,vol. 232, pp. 503- 506 ,(1986) , 10.1126/SCIENCE.2421410

S. M. Audigier, J. K. Wang, P. Greengard, Membrane depolarization and carbamoylcholine stimulate phosphatidylinositol turnover in intact nerve terminals Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 2859- 2863 ,(1988) , 10.1073/PNAS.85.8.2859

Kishio Furuya, Koh-ichi Enomoto, Sonoko Furuya, Shunichi Yamagishi, Charles Edwards, Takami Oka, Single calcium-activated potassium channel in cultured mammary epithelial cells. Pflügers Archiv: European Journal of Physiology. ,vol. 414, pp. 118- 124 ,(1989) , 10.1007/BF00580952

Roger Y. Tsien, New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. ,vol. 19, pp. 2396- 2404 ,(1980) , 10.1021/BI00552A018

M. J. Welsh, J. D. McCann, Intracellular calcium regulates basolateral potassium channels in a chloride-secreting epithelium Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 8823- 8826 ,(1985) , 10.1073/PNAS.82.24.8823

10.

H. Murakami, H. Masui, Hormonal control of human colon carcinoma cell growth in serum-free medium. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 3464- 3468 ,(1980) , 10.1073/PNAS.77.6.3464

Simultaneous Measur of Cell (Ca) and glr Explore Calcium's Role

来源期刊

我的账户

Simultaneous Measur of Cell (Ca) and glr Explore Calcium's Role

来源期刊

相似文章 0

我的账户