Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline
作者： Kaoru Sato , Yuka W. Iwasaki , Aoi Shibuya , Piero Carninci , Yuuta Tsuchizawa
DOI: 10.1016/J.MOLCEL.2015.06.024
关键词:
摘要: Piwi-interacting RNAs (piRNAs) suppress transposon activity in animal germ cells. In the Drosophila ovary, primary Aubergine (Aub)-bound antisense piRNAs initiate ping-pong cycle to produce secondary AGO3-bound sense piRNAs. This increases number of Aub-bound that can act destroy mRNAs. Here we show Krimper (Krimp), a Tudor-domain protein, directly interacts with piRNA-free AGO3 promote symmetrical dimethylarginine (sDMA) modification, ensuring piRNA-loading onto sDMA-modified AGO3. aub mutant ovaries, associates signature piRNAs, suggesting AGO3's compatibility piRNA loading. Krimp sequesters ectopically expressed within bodies cultured ovarian somatic cells (OSCs), which only pathway operates. Upon krimp-RNAi OSCs, loads further showing capacity for We propose enforces an bias on pools by binding and blocking its access
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