Effects of hyperthermia, irradiation, and cytotoxic drugs on fluorescein isothiocyanate staining intensity for flow cytofluorometry.

摘要: Measurement of fluorescein isothiocyanate (FITC) staining intensity cultured lymphoblastoid cells following hyperthermia showed large increases without concomitant in nuclear protein. Similar measurements incubation with cytotoxic drugs fluorescent that exceeded the protein were due to cell cycle blocking action drug. The reverse, however, was true for irradiation. In contrast, FITC and proceeding through after removal agent nearly parallel changes, although there reproducible minor differences, especially hydroxyurea. These results suggest is a function not only content but also stain access reaction sites constituents chromatin. Thus, it possible may be used as probe changes chromatin structure experimental manipulation vitro or treatment tumors vivo.