Animal models for arterial thrombolysis and prevention of reocclusion. Erythrocyte-rich versus platelet-rich thrombus.

摘要: Experimental animal models for erythrocyte-rich (ER) and platelet-rich (PR) arterial thrombosis were developed in dogs rabbits used the evaluation of effect antithrombin antiplatelet agents on thrombolysis with recombinant tissue-type plasminogen activators (rt-PA). The canine consist a whole blood clot produced left anterior descending coronary artery (ER thrombus) or 1-cm everted (inside-out) segment graft circumflex that predisposes to occlusion PR material (PR thrombus). rabbit femoral eversion are sensitive recanalization rt-PA but consistently associated reocclusion, notwithstanding concomitant use heparin and/or aspirin. Clot lysis is accelerated reocclusion prevented by administration F(ab')2 fragments monoclonal antibody 7E3 directed against platelet glycoprotein IIb/IIIa receptor; Argatroban, synthetic thrombin inhibitor; kistrin, IIb/IIIa-blocking polypeptide from Malayan pit viper venom. thrombus very resistant rt-PA, this resistance can be overcome antibody. Thus, selective inhibitors more effective than aspirin, heparin, both accelerating rt-PA; preventing after lysis; overcoming dispersion rt-PA. These experimental may useful development improved thrombolytic strategies using conjunction specifically targeted anticoagulant agents.