Antibodies to adenosine 5'-monophosphate: purification and specificity.

摘要: Antibodies to adenosine-5′-monophosphate were produced in rabbits by injecting a conjugate of the nucleotide (oxidized with periodate) bovine serum albumin. Nucleotide-specific antibodies isolated affinity chromatography on oligoadenylic acids/agarose column. Pure immunoglobulin G obtained gel filtration Sephadex G-200. These antibodies, as analyzed double diffusion react adenosine 5′-monophosphate-bovine albumin, slightly inosine-5′-monophosphate and not at all other conjugates. The association constants for adenosine-5′-monophosphate-antibody complex formation dialysis equilibrium fluorescence measurements, are good agreement. This latter technique was used study one hand influence temperature salt formation, interaction AMP derivatives. phosphate group, ribose base recognized antibody, but C-8 atom adenine residues is directly involved binding.