Antibodies to Poly(I) · poly(C)

摘要: Sera of rabbits immunized with poly(I) · poly(C) adsorbed to methylated serum albumin react poly(C), and poly(C). Three families antibodies have been isolated by affinity chromatography. The precipitate but neither nor In 0.15 M NaCl, pH 7.5 at 4°C the only in 10 mM NaCl room temperature they These reactions occur because destabilize behave as melting proteins. The thermal stability is decreased presence increased It has also found that lower tm transition poly(A) 2 poly(U) increase + poly(U). The purified several double-stranded polyribonucleotides such poly(U), poly(brC), poly(ioC), do not poly(G) poly(X) or DNA. Association constants for binding Fab fragments poly(l) poly(brC) deduced from fluorescence experiments. off ab quenched. analysis isotherms shows about 3–4 base pairs are covered site fragments, a value agreement precipitin curve (five pairs). From variation association function salt concentration it concluded two phosphate groups involved electrostatic interactions.