The molecular weight of a rabbit antibody.

摘要: Abstract Rabbit antibody, specific for the 2,4-dinitrophenyl group attached to lysine, exhibits a weak tendency associate at pH 7.4, 20°; its monomer molecular weight appears be 135 000 ± 5000. These conclusions are supported by detailed physicochemical studies of antibody. At protein concentration below 2 mg/ml, sedimentation coefficient, s 20, w , increased with concentration; higher concentrations, values showed more usual decrease but dependence was abnormally small. Data from equilibrium experiments, using Rayleigh optics 2–3-mm liquid columns solution, consistently yielded curvature in plots ln c concentration, versus x where is distance center rotation; heterogeneity thus indicated. Analysis these results terms dependance average weight, M on that as ( ) increased. The data were adequately described theoretical curve constructed dimerizing system 130 and association constant 0.04 l/g. wide scatter previously reported values, 137 000–188 000, can explained neglect protein's associate.