OC-09 - Microparticle-mediated transfer of TF hypercoagulability between cancer cells

摘要: Introduction Tissue Factor (TF) promotes apoptosis-resistance and facilitates haematogenous metastasis. Cancer cells release TF-bearing microparticles (TF-MP) which have pro-coagulant activity. TF-MPs from cancer induce increase in TF expression procoagulant activity of recipient endothelial cells. Drug resistance transfer between cells, resulting induction cell drug resistance, however has not been demonstrated. Aim To determine if isolated expressing lines can induced TF-mediated that do express TF. Materials Methods A7 (a melanoma line does TF) were transformed to with adenoviral transformation. In addition, the TF-expressing breast MDAMB231 was used. Media collected (1) wild-type (A7-WT) acted as control (2) (3) ‘low’ levels TF-adenovirus (4) ‘high’ TF-adenovirus. MPs media by centrifugation using standard techniques. Isolated incubated overnight. Pro-coagulant A7-WT assessed a FXa generation assay. Results i) overnight MDAMB231-derived produced an almost 5 fold compared (Mean 3.5 mOD/min S.E 0.32 vs 0.68 S.E. 0.05, p  Conclusions These results demonstrate, for first time, microparticle-mediated shed vivo implicated progression hypercoagulability. The acquisition spread MP-mediated implications biology cancer-induced