Biodiversity (Communications arising (reply)): Suspect evidence of transgenic contamination/Maize transgene results in Mexico are artefacts (see editorial footnote)

作者: David Quist , Ignacio H. Chapela

DOI: 10.1038/NATURE740

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摘要: Our original publication1 contained two separate conclusions derived from methodological approaches. First, using PCR, we detected the presence of three distinct transgenic DNA sequences in maize landraces Oaxaca, Mexico1. Second, attempted to establish genomic context transgene insertion i-PCR. The criticisms raised by Metz and Futterer Kaplinsky et al. relate principally our second statement.

参考文章(7)
Pieter Windels, Isabel Taverniers, Ann Depicker, Erik Van Bockstaele, Marc De Loose, Characterisation of the Roundup Ready soybean insert European Food Research and Technology. ,vol. 213, pp. 107- 112 ,(2001) , 10.1007/S002170100336
James C. Register, David J. Peterson, Philip J. Bell, W. Paul Bullock, Ian J. Evans, Bronwyn Frame, Andrew J. Greenland, Nicole S. Higgs, Ian Jepson, Shuping Jiao, Carol J. Lewnau, Jeff M. Sillick, H. Martin Wilson, Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment Plant Molecular Biology. ,vol. 25, pp. 951- 961 ,(1994) , 10.1007/BF00014669
W. P. Pawlowski, D. A. Somers, Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 12106- 12110 ,(1998) , 10.1073/PNAS.95.21.12106
Ajay Kohli, Daphrose Gahakwa, Philippe Vain, David A. Laurie, Paul Christou, Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing Planta. ,vol. 208, pp. 88- 97 ,(1999) , 10.1007/S004250050538
David Quist, Ignacio H. Chapela, Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico Nature. ,vol. 414, pp. 541- 543 ,(2001) , 10.1038/35107068
Vera Gorbunova, Avraham A Levy, Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions Nucleic Acids Research. ,vol. 25, pp. 4650- 4657 ,(1997) , 10.1093/NAR/25.22.4650