Intravesical Gene Therapy: In Vivo Gene Transfer Using Recombinant Vaccinia Virus Vectors

摘要: Intratumoral gene transfer may be a significant tool in active immunotherapy. The ability to insert functional genes into tumor vitro and vivo using recombinant vaccinia vectors was examined the murine bladder model. Vaccinia recombinants expressing influenza hemagglutinin or nucleoprotein antigens infected/transfected (MB-49 MBT-2) human (T24) cell lines . Systemic immunity induced with as few 10 plaque-forming units of instilled intravesically, encoded protein expressed urothelium. However, preimmunity did not inhibit intravesical transfection. Thus, virus is effective introducing foreign locally , supporting its use clinical