Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry.
作者： Stephen J. Juris , Kavita Shah , Kevan Shokat , Jack E. Dixon , Panayiotis O. Vacratsis
DOI: 10.1016/J.FEBSLET.2005.11.071
关键词:
摘要: Yersinia encodes a protein kinase, YpkA, which disrupts the actin cytoskeleton. Using an approach termed chemical genetics, we identified 36-kDa substrate for YpkA in both J774 lysates and bovine brain cytosol. Mass spectrometry analysis this as FLJ20113, open reading frame that corresponds to otubain 1, deubiquitinating enzyme implicated immune cell clonal anergy. We demonstrate 1 is phosphorylated by vitro interacts with vivo. Identification of suggests regulation anergy may be survival mechanism Yersinia.
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sci-hub.se 参考文章(12)
Wim Van Criekinge, Maria van Gurp, Els Decoster, Peter Schotte, Marc Van de Craen, Walter Fiers, Peter Vandenabeele, Rudi Beyaert, Use of the yeast three-hybrid system as a tool to study caspases. Analytical Biochemistry. ,vol. 263, pp. 62- 66 ,(1998) , 10.1006/ABIO.1998.2823
Luis Soares, Christine Seroogy, Heidi Skrenta, Niroshana Anandasabapathy, Patricia Lovelace, Chan D Chung, Edgar Engleman, C Garrison Fathman, Two isoforms of otubain 1 regulate T cell anergy via GRAIL. Nature Immunology. ,vol. 5, pp. 45- 54 ,(2004) , 10.1038/NI1017
S. J. Juris, A. E. Rudolph, D. Huddler, K. Orth, J. E. Dixon, A distinctive role for the Yersinia protein kinase: Actin binding, kinase activation, and cytoskeleton disruption Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 9431- 9436 ,(2000) , 10.1073/PNAS.170281997
Hasem Habelhah, Kavita Shah, Lan Huang, Alma L. Burlingame, Kevan M. Shokat, Ze'ev Ronai, Identification of New JNK Substrate Using ATP Pocket Mutant JNK and a Corresponding ATP Analogue Journal of Biological Chemistry. ,vol. 276, pp. 18090- 18095 ,(2001) , 10.1074/JBC.M011396200
Edouard E. Galyov, Sebastian Håkansson, Åke Forsberg, Hans Wolf-Watz, A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant. Nature. ,vol. 361, pp. 730- 732 ,(1993) , 10.1038/361730A0
Kira S Makarova, L Aravind, Eugene V Koonin, A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniae Trends in Biochemical Sciences. ,vol. 25, pp. 50- 52 ,(2000) , 10.1016/S0968-0004(99)01530-3
Anthony Bishop, Oleksandr Buzko, Stephanie Heyeck-Dumas, Ilyoung Jung, Brian Kraybill, Yi Liu, Kavita Shah, Scott Ulrich, Laurie Witucki, Feng Yang, Chao Zhang, Kevan M. Shokat, Unnatural ligands for engineered proteins: new tools for chemical genetics. Annual Review of Biophysics and Biomolecular Structure. ,vol. 29, pp. 577- 606 ,(2000) , 10.1146/ANNUREV.BIOPHYS.29.1.577
Mercedes POZUELO RUBIO, Kathryn M. GERAGHTY, Barry H. C. WONG, Nicola T. WOOD, David G. CAMPBELL, Nick MORRICE, Carol MACKINTOSH, 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking. Biochemical Journal. ,vol. 379, pp. 395- 408 ,(2004) , 10.1042/BJ20031797
K. Shah, Y. Liu, C. Deirmengian, K. M. Shokat, Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 3565- 3570 ,(1997) , 10.1073/PNAS.94.8.3565
Stephen J. Juris, Feng Shao, Jack E. Dixon, Yersinia effectors target mammalian signalling pathways. Cellular Microbiology. ,vol. 4, pp. 201- 211 ,(2002) , 10.1046/J.1462-5822.2002.00182.X