MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics.
作者： Kelin Wang , Fabrizio Donnarumma , Michael E. Pettit , Carson W. Szot , Touradj Solouki
DOI: 10.1002/JMS.4475
关键词:
摘要: A multimodal workflow for mass spectrometry imaging was developed that combines MALDI with protein identification and quantification by liquid chromatography tandem (LC-MS/MS). Thin tissue sections were analyzed imaging, the regions of interest (ROI) identified using a smoothing edge detection procedure. midinfrared laser at 3-μm wavelength used to remove ROI from brain section after (MALDI MSI). The captured material processed single-pot solid-phase-enhanced sample preparation (SP3) method LC-MS/MS ion mobility (IM) enhanced data independent acquisition (DIA) identify quantify proteins; more than 600 proteins identified. Using modified database included isoform post-translational modifications chain, loss initial methionine, acetylation, 14 MSI peaks Comparison Kyoto Encyclopedia Genes Genomes (KEGG) pathways achieved through an evolutionary relationships classification system.
harvard.edu
sci-hub.se 参考文章(71)
Chandrakant Tayade, Andrew K. Edwards, Mallikarjun Bidarimath, Laser Capture Microdissection The Guide to Investigation of Mouse Pregnancy. pp. 567- 575 ,(2014) , 10.1016/B978-0-12-394445-0.00048-5
Scott Chaffee, Sashwati Roy, Ryan Dickerson, Chandan K. Sen, Somak Datta, Lavina Malhotra, Laser capture microdissection: Big data from small samples Histology and Histopathology. ,vol. 30, pp. 1255- 1269 ,(2015) , 10.14670/HH-11-622
Xinhua Zhan, Glen C. Jickling, Bradley P. Ander, Boryana Stamova, DaZhi Liu, Patricia F. Kao, Mariko A. Zelin, Lee-Way Jin, Charles DeCarli, Frank R. Sharp, Myelin basic protein associates with AβPP, Aβ1-42, and amyloid plaques in cortex of Alzheimer's disease brain. Journal of Alzheimer's Disease. ,vol. 44, pp. 1213- 1229 ,(2015) , 10.3233/JAD-142013
Sung-Gun Park, Kermit K. Murray, Infrared laser ablation sample transfer for on-line liquid chromatography electrospray ionization mass spectrometry. Journal of Mass Spectrometry. ,vol. 47, pp. 1322- 1326 ,(2012) , 10.1002/JMS.3096
Fabrizio Donnarumma, Fan Cao, Kermit K. Murray, Laser Ablation with Vacuum Capture for MALDI Mass Spectrometry of Tissue Journal of the American Society for Mass Spectrometry. ,vol. 27, pp. 108- 116 ,(2016) , 10.1007/S13361-015-1249-0
Henrik Lodén, Mohammadreza Shariatgorji, Anna Nilsson, Per E Andrén, An introduction to MS imaging in drug discovery and development Bioanalysis. ,vol. 7, pp. 2621- 2627 ,(2015) , 10.4155/BIO.15.186
Jusal Quanico, Julien Franck, Claire Dauly, Kerstin Strupat, Jocelyn Dupuy, Robert Day, Michel Salzet, Isabelle Fournier, Maxence Wisztorski, Development of liquid microjunction extraction strategy for improving protein identification from tissue sections. Journal of Proteomics. ,vol. 79, pp. 200- 218 ,(2013) , 10.1016/J.JPROT.2012.11.025
Lenka Munoz, Alaina J. Ammit, Targeting p38 MAPK pathway for the treatment of Alzheimer's disease. Neuropharmacology. ,vol. 58, pp. 561- 568 ,(2010) , 10.1016/J.NEUROPHARM.2009.11.010
Mado Vandewoestyne, Karen Goossens, Christian Burvenich, Ann Van Soom, Luc Peelman, Dieter Deforce, Laser capture microdissection: should an ultraviolet or infrared laser be used? Analytical Biochemistry. ,vol. 439, pp. 88- 98 ,(2013) , 10.1016/J.AB.2013.04.023
Joaquim V. Marques, Doralyn S. Dalisay, Hong Yang, Choonseok Lee, Laurence B. Davin, Norman G. Lewis, A multi-omics strategy resolves the elusive nature of alkaloids in Podophyllum species Molecular BioSystems. ,vol. 10, pp. 2838- 2849 ,(2014) , 10.1039/C4MB00403E