A system for the measurement of gene targeting efficiency in human cell lines using an antibiotic resistance-GFP fusion gene.

作者: Yuko Konishi , Sivasundaram Karnan , Miyuki Takahashi , Akinobu Ota , Lkhagvasuren Damdindorj

DOI: 10.2144/0000113911

关键词:

摘要: Gene targeting in a broad range of human somatic cell lines has been hampered by inefficient homologous recombination. To improve this technology and facilitate its widespread application, it is cr...

参考文章(38)
Nicholas Muzyczka, Dietmar W. Siemann, Kenneth H. Warrington, Christian Teschendorf, Comparison of the EF-1 alpha and the CMV promoter for engineering stable tumor cell lines using recombinant adeno-associated virus. Anticancer Research. ,vol. 22, pp. 3325- 3330 ,(2002)
Sairei So, Yuji Nomura, Noritaka Adachi, Yuki Kobayashi, Tamaki Hori, Yasuyuki Kurihara, Hideki Koyama, Enhanced gene targeting efficiency by siRNA that silences the expression of the Bloom syndrome gene in human cells Genes to Cells. ,vol. 11, pp. 363- 371 ,(2006) , 10.1111/J.1365-2443.2006.00944.X
Maria L. Arbonés, Harry A. Austin, Daniel J. Capon, Gary Greenburg, Gene targeting in normal somatic cells: inactivation of the interferon–γ receptor in myoblasts Nature Genetics. ,vol. 6, pp. 90- 97 ,(1994) , 10.1038/NG0194-90
J. Yun, C. Rago, I. Cheong, R. Pagliarini, P. Angenendt, H. Rajagopalan, K. Schmidt, J. K. V. Willson, S. Markowitz, S. Zhou, L. A. Diaz, V. E. Velculescu, C. Lengauer, K. W. Kinzler, B. Vogelstein, N. Papadopoulos, Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells Science. ,vol. 325, pp. 1555- 1559 ,(2009) , 10.1126/SCIENCE.1174229
Anton A Komar, Maria Hatzoglou, Cellular IRES-mediated translation: the war of ITAFs in pathophysiological states. Cell Cycle. ,vol. 10, pp. 229- 240 ,(2011) , 10.4161/CC.10.2.14472
P. F. Swann, T. R. Waters, D. C. Moulton, Y.-Z. Xu, Q. Zheng, M. Edwards, R. Mace, Role of Postreplicative DNA Mismatch Repair in the Cytotoxic Action of Thioguanine Science. ,vol. 273, pp. 1109- 1111 ,(1996) , 10.1126/SCIENCE.273.5278.1109
J. M. Sedivy, P. A. Sharp, Positive genetic selection for gene disruption in mammalian cells by homologous recombination Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 227- 231 ,(1989) , 10.1073/PNAS.86.1.227
LISA O. ROBERTS, RACHAEL A. SEAMONS, GRAHAM J. BELSHAM, Recognition of picornavirus internal ribosome entry sites within cells; influence of cellular and viral proteins. RNA. ,vol. 4, pp. 520- 529 ,(1998) , 10.1017/S1355838298971989
Hiroyuki Konishi, Josh Lauring, Joseph P Garay, Bedri Karakas, Abde M Abukhdeir, John P Gustin, Yuko Konishi, Ben H Park, A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting Nature Protocols. ,vol. 2, pp. 2865- 2874 ,(2007) , 10.1038/NPROT.2007.409