Angiogenesis inhibitor TNP-470 inhibits murine cutaneous wound healing.

摘要: Background.TNP-470 (AGM-1470) is a potent inhibitor of angiogenesis with potential therapeutic applications in neoplastic and angio-proliferative diseases. This study evaluated its effect on cutaneous wound healing murine dorsal excisional model. Materials methods.Full-thickness wounds (1.60 cm2) were created the dorsum homozygous/hairless mice (7 to 9 weeks). Wound areas measured alternate days for 16 days. Experimental groups consisted (1) TNP-470 administered doses 0.05, 0.5, 5.0 mg/kg Days 0, 2, 4 or 0 through 6; (2) (5.0 mg/kg) coadministered minocycline (4.0 10 4; (3) 4) topical basic fibroblast growth factor (bFGF) 1.0 μg/wound 1, 2. Hematoxylin eosin staining was used compare experimental control wounds. Results.TNP-470 administration significantly decreased dose-dependent manner versus controls (P< .05). The concentration yielded greatest by maintaining an average area 20.4% greater than marked delay H&E staining. Alternate-day dosing as effective consecutive day administration. Minocycline did not augment inhibition TNP-470. Coadministration bFGF eliminated any rate-altering upon resulted similar controls. Conclusion.Therapy induces significant healing. may be exploited use situations where excessive debilitating. Topical application can overcome TNP-470-induced inhibition.