The extensive and condition-dependent nature of epistasis among whole-genome duplicates in yeast

摘要: Since complete redundancy between extant duplicates (paralogs) is evolutionarily unfavorable, some degree of functional congruency eventually lost. However, in budding yeast, experimental evidence collected for duplicated metabolic enzymes and global physical interaction surveys had suggested widespread overlap paralogs. While maintained thought to confer robustness against genetic mutation facilitate environmental adaptability, it has yet be determined what properties define paralogs that can compensate the phenotypic consequence deleting a sister gene, how extensive this epistasis is, adaptable toward alternate states. To end, we have performed comprehensive analysis as indicated by aggravating interactions resulting from an ancient whole-genome duplication (WGD) event occurring yeast Saccharomyces cerevisiae, thus were able compare large numbers epistatic non-epistatic with identical evolutionary times since divergence. We found more than one-third (140) 399 examinable WGD paralog pairs under standard laboratory conditions additional cases became obvious only media designed induce cellular stress. Despite significant increase within-species sequence co-conservation, protein revealed not functionally overlapping those non-epistatic. As impact on categorization deemed fraction potential stress been interrogated here, hypothesize many relationships remain unresolved.