Method for selecting recombinase variants with altered specificity

摘要: Disclosed are variants of Cre recombinase that have broadened specificity for the site recombination. Specifically, disclosed mediate recombination between sequences other than loxP sequence and lox on which wild type is active. In general, efficient sites can act (referred to as sites), variant not efficiently utilized by a site. Also methods recombining nucleic acids using variants. For example, be used in any method or technique where (or other, similar recombinases such FLP) used. addition, allow different alternative recombinations performed since much more sitsites sites. Control accomplish sophisticated sequential achieve results possible with recombinase.