Mice transgenic for a tetracycline-controlled transcriptional activator

摘要: Transgenic mice carrying two transgenes, the first coding for a transactivator fusion protein comprising tet repressor and polypeptide which directly or indirectly activates transcription of operator-linked gene in eucaryotic cells, second operably linked to minimal promotor at least one operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) integrating polynucleotide sequence encoding invention predetermined location within target molecule by homologous recombination also having integrated chromosome encompassed invention. Methods regulate expression linked-gene interest administering tetracycline analogue mouse The regulatory system allows conditional inactivation modulation host cell mouse.