High efficiency regulatable gene expression system

摘要: Highly efficient regulated promoters (RPs) and transactivators are provided. The RPs contain 2-8 transactivator binding domains positioned in an optimized manner with respect to each other. In a tetracycline-regulatable system, the RP displays 5- 10-fold enhanced regulation efficiency by maintaining high maximal expression while offering reduced basal leakiness. transient studies, these novel display over 900-fold gene at 1:1 ratio of reporter plasmid. Furthermore, preserve their regulator efficienty context single positive feedback regulatory vector that presents ease delivery system for many uses, including use vivo therapy. Finally, humanized provided, example tetracycline utilizing human transactivational domain NF-λ p65 protein fused repressor (terR) is provided functions as efficiently tetracycline-regulated (tTA) should reduce potential immunogenicity original tTA.