Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units
作者： Manfred Gossen , Hermann Bujard
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摘要: Nucleic acid molecules and proteins useful for regulating the expression of genes in eukaryotic cells organisms an inducible manner are disclosed. In regulatory system invention, transcription a tet operator-linked nucleotide sequence is stimulated by transcriptional activator fusion protein composed two polypeptides, first polypeptide which binds to operator sequences presence tetracycline or analogue second directly indirectly activates cells. one embodiment, comprises mutated Tet repressor operatively linked activation polypeptide, such as portion herpes simplex virus virion 16. absence inducing agent (tetracycline analogue), remains uninduced. agent, transactivator invention. Novel units allow coordinate independent tetracycline-regulated more invention also Kits including components encompassed
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