Mice transgenic for a tetracycline-inducible transcriptional activator
作者： Hermann Bujard , Manfred Gossen
DOI:
关键词: Fusion protein 、 Molecular biology 、 Genetically modified mouse 、 Activator (genetics) 、 Gene 、 Transgene 、 Biology 、 Nucleic acid sequence 、 Transcription (biology) 、 Tetracycline
摘要: Transgenic mice carrying a transgene comprising nucleic acid molecule encoding protein useful for regulating the expression of genes in eukaryotic cells highly controlled manner are disclosed. In regulatory system invention, transcription tet operator-linked nucleotide sequence is stimulated by transcriptional activator fusion composed two polypeptides, first polypeptide which binds to operator sequences presence tetracycline operatively linked second activates cells. preferred embodiment, integrated at predetermined location within chromosome transgenic mouse.
lens.org 参考文章(84)
A Wimmel, F C Lucibello, R Müller, A Sewing, S Adolph, Inducible acceleration of G1 progression through tetracycline-regulated expression of human cyclin E Oncogene. ,vol. 9, pp. 995- 997 ,(1994)
A. Baniahmad, A.C. Köhne, R. Renkawitz, A transferable silencing domain is present in the thyroid hormone receptor, in the v-erbA oncogene product and in the retinoic acid receptor. The EMBO Journal. ,vol. 11, pp. 1015- 1023 ,(1992) , 10.1002/J.1460-2075.1992.TB05140.X
Sherry C. Cipriano, Julie M. Arenkiel, Yong Q. Chen, Fred R. Miller, Tumor Suppression by p21WAF1 Cancer Research. ,vol. 55, pp. 4536- 4539 ,(1995)
Melvin Silberklang, Iii George E. Mark, Ming-Fan Law, Chung-Her Jenh, Mammalian inducible promoter cascade system ,(1991)
Betty Slagle, Janet S. Butel, Allan Bradley, Lawrence A. Donehower, Tumor susceptible non-human animals ,(1992)
Alexander Hollaender, Jane K. Setlow, Genetic Engineering: Principles and Methods ,(1979)
L. Altschmied, R. Baumeister, K. Pfleiderer, W. Hillen, A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT. The EMBO Journal. ,vol. 7, pp. 4011- 4017 ,(1988) , 10.1002/J.1460-2075.1988.TB03290.X
Frank H. Ruddle, Guerard W. Byrne, Multiplex gene regulation ,(1988)
B Hecht, G Müller, W Hillen, Noninducible Tet repressor mutations map from the operator binding motif to the C terminus. Journal of Bacteriology. ,vol. 175, pp. 1206- 1210 ,(1993) , 10.1128/JB.175.4.1206-1210.1993