Somatostatin inhibits secretin-induced ductal hypercholeresis and exocytosis by cholangiocytes.

摘要: Previous work from our laboratory has implicated hormone-induced plasma membrane movement (i.e., endo- and exocytosis) in water electrolyte transport by the epithelial cells that line ducts liver cholangiocytes). To further explore cellular mechanisms regulating ductal bile secretion, we infused somatostatin and/or secretin intravenously into rats 2 wk after either duct ligation (BDL), a procedure induces selective proliferation of cholangiocytes, or sham surgery measured flow biliary constituents. We also determined effect on basal secretin-induced exocytosis purified cholangiocytes isolated rat BDL. Finally, studied expression receptor gene both ribonuclease (RNase) protection nuclear run-on assays using cDNA encoding for two subtypes SSTR1 SSTR2). In vivo, infusion caused dose-dependent bicarbonate-poor decrease (57% m...