Molecular Role of Reactive Oxygen Species in Photoaging and Tumor Progression

摘要: The increase in UV irradiation on earth due to the stratospheric ozone depletion represents a major environmental threat skin increasing its risk of photooxidative damage by reactive oxygen species (ROS). ROS have been implicated several photodermatological disorders including photoaging. clinical manifestations cutaneous photoaging are quantitative and qualitative alterations dermal connective tissue. Therefore, we were interested better define involvement up-regulation matrix-metalloproteinases (MMP) responsible for tissue degradation photoaging, tumor invasion metastasis. For this purpose fibroblast monolayer cultures subjected various modalities. In parallel fibroblasts challenged distinct generating systems. Using non-toxic concentrations scavengers, enhancers, chemicals which specifically inhibit activities detoxifying enzymes, stably transfected cell lines overexpressing iron chelators blocking Fenton reaction, increased perior intracellularly. A time dose dependent mRNA protein levels collagenase was observed after exposure UVB, UVA, 1O2, H2O2, O2, or OH After UVB inhibition reaction using abrogated MMP induction substantially. Scavengers hydroxyl radical reduced thus pointing importance response. By modulating lifetime 1O2 enhancers quenchers, singlet could be identified as crucial mediator UVA addition, glutathione peroxidase, catalase enhanced whereas superoxide dismutase diminished increase. Furthermore, line manganese resulted substantial mRNA. These results indicate that following irradiation, beside H2O2 is mainly synthesis. conclusion, involved matrix-degrading enzyme resulting outlined preventive strategies may enhance rational development photoprotective agents.