Epidermal Growth Factor and Its Receptor in Bovine Corpus Luteum during the Estrous Cycle

摘要: The epidermal growth factor (EGF) concentration and its binding characteristics in bo-vine corpus luteum (CL) during the estrous cycle (early: Days 3-5, mid-: 8-12, late stage: 15-18) were determined. EGF concentrations tissue, evaluated by a radioreceptor assay using human placenta, 3.0 ± 0.7 ng/g early luteal phase, 43.9 2.2 mid-luteal phase 18.6 2.7 phase. Specific receptors present bovine of all phases. TGFα, but not IGF-I, FGF NGF competitively displaced binding. affinity differed between stages decreased significantly from (Kd=0.8 0.04 nM) to stage (Kd=1.6 0.34 nM, P<0.05). capacity receptor at different similar (early stage; Bmax=35.1 5.7 fmol/mg protein, Bmax=46.3 4.0 values mean SEM). These results suggest that plays physiological role for regulation function cycle.