Tetrahydrobiopterin, l-Arginine and Vitamin C Act Synergistically to Decrease Oxidative Stress, Increase Nitric Oxide and Improve Blood Flow after Induction of Hindlimb Ischemia in the Rat

摘要: Nitric oxide (NO) derived from endothelial nitric synthase (eNOS) is a potent vasodilator and signaling molecule that plays an essential role in vascular remodeling of collateral arteries perfusion recovery response to hindlimb ischemia. In ischemic conditions, decreased NO bioavailability was observed because increased oxidative stress, L-arginine tetrahydrobiopterin. This study tested the hypothesis dietary cosupplementation with tetrahydrobiopterin (BH4), L-arginine, vitamin C acts synergistically decrease increase improve blood flow acute Rats were fed normal chow, chow supplemented BH4 or (alone combination) + for 1 wk before induction unilateral Cosupplementation resulted greater eNOS expression, Ca2+-dependent NOS activity concentration gastrocnemius hindlimb, as well foot more artery enlargement than did rats receiving either agent separately. The addition regimen further these dependent variables, although only expression reached statistical significances. addition, given all three supplements demonstrated significantly less Ca2+-independent activity, nitrotyrosine accumulation, glutathione:glutathione disulfide (GSH:GSSG) ratio muscle necrosis, on both macroscopic microscopic levels. conclusion, co-supplementation after ischemia by increasing reducing stress tissue necrosis. Oral holds promise biological therapy induce enlargement.