Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units

摘要: The PHA biosynthetic pathway is combined with a succinic semialdeyde metabolic that metabolizes semialdhyde via 4-hydroxybutyryl-CoA intermediate in order to produce high levels of comprising 4HB monomer units. This combination particularly useful, part, because the well known and has been expressed P(3HB) as 70-80 % cell dry weight, while metalbolic also known, two pathways means production PHAs units does not depend on immediate precursors 4-hydroxybutyrate. Rather, can occur simply from extraction succinate or succinyl-CoA citric acid cycle when host grown an inexpensive carbon source such glucose.