Properties of immunoreactive glucagon fractions of canine stomach and pancreas.

摘要: Abstract The present study was designed to identify the physicochemical, immunologic, and biologic properties of immunoreactive glucagon (IRG) moieties canine gastric fundus compare them with those pancreas. Acid-alcohol extracts pancreas dogs were subjected Bio-Gel P-10 chromatography, elution profiles both organs revealed IRG peaks in Mr = 2,000 3,500, 9,000 zones; extracts, a void volume peak also present. On basis Sephadex G-150 rechromatography sucrose density gradient ultracentrifugation latter estimated have 65,000. Incubation fundic IRG65,000 8 M urea failed alter its position. Its pI 6.4, while IRG3,500 had 6.15 pancreatic 6.25.Fundic IRG9,000 4.5 4.65. Dilution curves these three two IRGs parallel crystalline beef-pork glucagon. glycogenolytic activity IRG65,000, measured isolated rat liver system, not different from that immunoequivalent amounts dog or Both devoid lacker adenylate cyclase stimulating 125I-glucagon displacing when tested on partially purified membranes. Fundic however, stimulated displaced same degree as more active than activity. This clearly attributable differences binding cell