Colony to colorimetry in 6 h: ELISA detection of a surface-expressed Pseudomonas aeruginosa virulence factor using immobilized bacteria.

作者: Azmi Adawi , Lewis F. Neville

DOI: 10.1016/J.DIAGMICROBIO.2012.05.016

关键词:

摘要: A rapid ELISA employing intact Pseudomonas aeruginosa (PA) is described that allows discrimination between strains harboring flagellin type a or b. All 52 PA known to harbor b were positive in this when screened with fully human monoclonal antibody (LST-007) targeting Completion of assay only 6 h, from picking single bacterial colony colorimetric product, could easily be adapted clinical laboratory setting and permit the appropriate choice therapeutic versus its homologous target PA-infected patients.

参考文章(23)
T. C. Montie, T. R. Anderson, Enzyme-linked immunosorbent assay for detection of Pseudomonas aeruginosa H (flagellar) antigen. European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 7, pp. 256- 260 ,(1988) , 10.1007/BF01963097
K Kelly-Wintenberg, T C Montie, Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli. Journal of Bacteriology. ,vol. 171, pp. 6357- 6362 ,(1989) , 10.1128/JB.171.11.6357-6362.1989
P A Totten, S Lory, Characterization of the type a flagellin gene from Pseudomonas aeruginosa PAK. Journal of Bacteriology. ,vol. 172, pp. 7188- 7199 ,(1990) , 10.1128/JB.172.12.7188-7199.1990
James A Driscoll, Steven L Brody, Marin H Kollef, The Epidemiology, Pathogenesis and Treatment of Pseudomonas aeruginosa Infections Drugs. ,vol. 67, pp. 351- 368 ,(2007) , 10.2165/00003495-200767030-00003
Hongmei Yu, Xiangdong Zhou, Sha Wen, Qian Xiao, Flagellin/TLR5 responses induce mucus hypersecretion by activating EGFR via an epithelial cell signaling cascades. Experimental Cell Research. ,vol. 318, pp. 723- 731 ,(2012) , 10.1016/J.YEXCR.2011.12.016
T. Pressler, C. Bohmova, S. Conway, S. Dumcius, L. Hjelte, N. Høiby, H. Kollberg, B. Tümmler, V. Vavrova, Chronic Pseudomonas aeruginosa infection definition: EuroCareCF Working Group report Journal of Cystic Fibrosis. ,vol. 10, ,(2011) , 10.1016/S1569-1993(11)60011-8
Shigeki Fujitani, Hsin-Yun Sun, Victor L. Yu, Jeremy A. Weingarten, Pneumonia Due to Pseudomonas aeruginosa: Part I: Epidemiology, Clinical Diagnosis, and Source Chest. ,vol. 139, pp. 909- 919 ,(2011) , 10.1378/CHEST.10-0166
L. Liaudet, A. Deb, P. Pacher, J.G. Mabley, K.G. Murthy, A.L. Salzman, C. Szabo, The flagellin-TLR5 axis: Therapeutic opportunities Drug News & Perspectives. ,vol. 15, pp. 397- 409 ,(2002) , 10.1358/DNP.2002.15.7.840075
Lynette Cegelski, Garland R. Marshall, Gary R. Eldridge, Scott J. Hultgren, The biology and future prospects of antivirulence therapies Nature Reviews Microbiology. ,vol. 6, pp. 17- 27 ,(2008) , 10.1038/NRMICRO1818