Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli.

摘要: Abstract The flagellin gene was isolated from a Pseudomonas aeruginosa PAO1 genomic bank by conjugation into PA103 Fla- strain. Flagellin DNA transferred motile recipient Fla+ cells transformation Escherichia coli. We show that transformed E. coli expresses protein. Export of to the cell surface suggested positive colony blots unlysed and isolation protein supernatants.