Macrophage subpopulations in rheumatoid synovium: Reduced CD163 expression in CD4+ T lymphocyte-rich microenvironments
作者： J. E. Fonseca , J. C. W. Edwards , S. Blades , N. J. Goulding
DOI: 10.1002/ART.10207
关键词:
摘要: Objective The cell surface glycoprotein CD163 is a member of the cysteine-rich scavenger receptor family, highly specific for leukocytes mononuclear phagocyte lineage. In vitro, it induced by glucocorticoids, interleukin-6 (IL-6), and IL-10 down-regulated interferon-γ (IFNγ), indicating that has role in antiinflammatory or other immunomodulatory pathways. We assessed expression microenvironments within rheumatoid arthritis (RA) synovium to clarify relationships among CD4+ T lymphocytes, IFNγ, macrophage function RA. Methods Double immunofluorescence serial immunoenzymatic studies were performed on normal, osteoarthritic, RA tonsil with antibodies CD163, CD45, CD68, CD14, CD3, CD4, CD8, CD19, IFNγ. Results CD163 was observed all CD14+ cells exception larger lymphocyte clusters tonsillar follicles. All brightly around vessel walls (interpreted as immigrant monocytes) CD163+. labeled fewer than did CD68 synovial intima, but CD45+ intimal CD4+,IFNγ+ lymphocytes chiefly localized containing CD68+, CD163− cells. Conclusion Within synovium, major advantages marker does not appear be restricted “mature” macrophages. discriminates between macrophages fibroblasts, which also stain positively diseased tissue.
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