Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis

摘要: Androgens are essential for sexual development and reproduction. However, androgen regulation in health disease is poorly understood. We showed that human adrenocortical H295R cells grown under starvation conditions acquire a hyperandrogenic steroid profile with changes metabolizing enzymes HSD3B2 CYP17A1 production. Here we studied the regulatory mechanisms underlying production starved cells. Microarray expression profiling of normal versus revealed fourteen differentially expressed genes; HSD3B2, HSD3B1, CYP21A2, RARB, ASS1, CFI, ASCL1 ENC1 play role energy metabolism ANGPTL1, PLK2, DUSP6, DUSP10 FREM2 involved signal transduction. discovered two new gene networks around RARB show how they regulate biosynthesis. Transcription factor stimulated promoters genes (StAR, HSD3B2) enhanced androstenedione For worked cooperation Nur77. Secretory protein ANGPTL1 modulated DUSP6 by inducing ERK1/2 phosphorylation. By contrast, our studies no evidence hormones or cell cycle involvement regulating In summary, these establish firm