Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects

摘要: Recent advances of the research on synergistic effect a mixed baculovirus infection demonstrated presence viral molecules enhancing early event infection. The enhancins from Trichoplusia ni was identified to have such function, i.e., disrupting structural integrity peritrophic membrane midgut T. larva. enhancin gene ligated downstream CaMV 355 promoter binary vector pBI121. With drug resistant gene, introduced piece tobacco leaf, Nicotiana tobacum cv. Havana SR1. We screened 11 regenerated plants out 37 by feeding powder in artificial diet, 3rd instar Pseudaletia separata larvae. larval stage usually delayed 1 3 days comparison that control larvae did not pupate normally. showed irregular morphology half larva and pupae, suggesting hormonal disturbance caused transgenic tobacco. introduction an into is effective method protecting them insects due disruption their normal life cycle.