Capillary Zone Electrophoresis for the Diagnosis of Congenital Hemoglobinopathies

摘要: The analysis of human hemoglobins (Hbs) is medical importance in a number congenital defects. hemoglobinopathies are grouped into defective variants Hb, such as Hb S and >600 other variants, thalassemias characterized by abnormal expression the genes for normal globin chains (1)(2)(3). Alkaline electrophoresis, performed historically on cellulose acetate currently agarose, combined with citrate agar electrophoresis at acidic pH still widely used (4)(5)(6)(7), but high-performance cation-exchange chromatography (HPCEC) offers superior resolution, speed, automation (8)(9)(10). Capillary uses numerous separation principles shares HPLC advantages high resolution automation, on-line detection direct quantification (11)(12)(13)(14)(15). isoelectric focusing coated capillaries can be to study (16)(17)(18)(19)(20)(21), slower than HPCEC (21). first reported assays based capillary zone (CZE) gave poor Hbs or were not quantitative (22)(23)(24). aim present work was evaluate rapid CZE assay dynamic coating fused-silica alkaline detect quantify diagnosis thalassemias, confirm identity variants. Reagents obtained from Analis. kit A2 contained hemolyzer, an initiator consisting polycation, arginine buffer (pH 8.8) containing polyanion. A1 has been described elsewhere A1c measurement (25). Adult newborn samples collected EDTA-containing tubes maternity unit Hopital Saint-Antoine (AP-HP, Paris, France) received laboratory analysis. Whole blood …