In Vivo Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components
作者： Masataka Yoshikawa , Hideyuki Kakigi , Ayano Miyamoto , Sadaomi Sugimoto , Keisuke Nakai
DOI: 10.4236/JBISE.2016.911045
关键词:
摘要: A bi-phasic scaffold consisting of a columnar formaldehyde-acetalized polyvinyl alcohol (PVF) sponge and cylindrical porous hydroxyapatite (HA) with hollow center was devised. Rat bone marrow cells (rBMCs) were seeded into the placed in HA. The scaffold, HA PVF separated from after rBMC seeding, without rBMCs as negative control, implanted for 6 weeks rat dorsal subcutaneous tissue. In each construct, formation examined histologically osteocalcin measured immunochemically. Bone observed also isolated scaffold. significant difference quantity between No found sponge. an outer layer seemed to inhibit outflow This type may have two specific characteristics: Attachment both
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