PU.1-Regulated Long Noncoding RNA lnc-MC Controls Human Monocyte/Macrophage Differentiation through Interaction with MicroRNA 199a-5p.

摘要: Long noncoding RNAs (lncRNAs) are emerging as important regulators in mammalian development, but little is known about their roles monocyte/macrophage differentiation. Here we identified a long monocytic RNA (lnc-MC) that exhibits increased expression during differentiation of THP-1 and HL-60 cells well CD34(+) hematopoietic stem/progenitor (HSPCs) transcriptionally activated by PU.1. Gain- loss-of-function assays demonstrate lnc-MC promotes HSPCs. Mechanistic investigation reveals acts competing endogenous to sequester microRNA 199a-5p (miR-199a-5p) alleviate repression on the activin A receptor type 1B (ACVR1B), an regulator We also noted repressive effect miR-199a-5p function, PU.1-dominant downregulation weakens role reciprocal regulation between lnc-MC. Altogether, our work demonstrates two PU.1-regulated RNAs, miR-199a-5p, have opposing facilitates process, enhancing PU.1, soaking up releasing ACVR1B expression. Thus, reveal novel regulatory mechanism, comprising lnc-MC, ACVR1B,