Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis.
作者： Toshiaki Iba , Jerrold H. Levy , Tatsuhiko Hirota , Makoto Hiki , Koichi Sato
DOI: 10.1016/J.THROMRES.2018.09.042
关键词:
摘要: Abstract Introduction Injury and loss of the endothelial glycocalyx occur during early phase sepsis. We previously showed that antithrombin has a protective effect on this structure in vitro. Here, we investigated possible effects an animal model Methods Wistar rats were injected with endotoxin, circulating levels syndecan-1, hyaluronan, albumin, lactate other biomarkers measured antithrombin-treated group untreated control (n = 6 each group). Intravital microscopy was used to observe leukocyte adhesion, microcirculation, syndecan-1 staining. Results The hyaluronan significantly reduced group, compared controls. Lactate albumin reduction attenuated group. microscopic observation revealed both adhesion blood flow better maintained treatment lining disrupted after endotoxin treatment, derangement by antithrombin. Conclusion Antithrombin effectively microcirculation vascular integrity protecting rat sepsis model.
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