Expression of DNA-dependent Protein Kinase and Its Relationship with Epidermal Growth Factor Receptor Signaling in Metastatic Cancer Cell Lines

摘要: The genetic instability of cancer cells may be related to inappropriately activated DNA repair pathways. In present study, the modulated expression DNA-dependent protein kinase (DNA-PK), a major protein, in human metastatic was tested. expressions Ku70/80, regulatory subunit DNA-PK, and Ku DNA-binding activity various highly cell lines were higher than those each parental line. Also, DNA-PKcs, catalytic whole DNA-PK complex significantly increased as compared cells, suggesting that enhanced capacity could associated with aberrant use repair, which mediate tumor progression potential. Increased EGFR (epidermal growth factor receptor) signaling has been invasion metastasis, linkage between EGFR-mediated suggested. This study showed PKI166, new tyrosine inhibitor, Ku70/80 DNA-PKcs also revealed chemosensitization effect PKI166 against part due inhibition Ku70/80. These results suggest interference by inhibitor resulted impairment activity, thus possible molecular targets for therapy cells.