Hyaluronic acid suppresses the reduction of alpha2(VI) collagen gene expression caused by interleukin-1beta in cultured rabbit articular chondrocytes.
作者： Hiromasa Goto , Tomohiko Onodera , Hiroko Hirano , Tadashi Shimamura
DOI: 10.1620/TJEM.187.1
关键词:
摘要: In order to investigate how alpha2(VI) collagen gene is regulated by inflammatory cytokines in cultured rabbit articular chondrocytes, we examined the effect of interleukin-1beta (IL-1beta) on this mRNA expression. Polylayer cultures chondrocytes were exposed IL-1beta (0.1, 1, 10 ng/ml). Quantitative detection specific for was carried out reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, hyaluronic acid (HA) expression (10 ng/ml) presence HA (0.01, 0.1, 1 mg/ml) with molecular weight 900 kDa. Chondrocytes also (1 weights 200, and 2000 Alpha2(VI) decreased significantly ng/ml IL-1beta. However, addition both or all suppressed these reduced levels. No tendency suppression depend observed. These results suggest that transcriptional activity type VI will be associated reduction cartilage matrix tissue
sci-hub.se 参考文章(22)
B Saitta, D G Stokes, H Vissing, R Timpl, M L Chu, Alternative splicing of the human alpha 2(VI) collagen gene generates multiple mRNA transcripts which predict three protein variants with distinct carboxyl termini. Journal of Biological Chemistry. ,vol. 265, pp. 6473- 6480 ,(1990) , 10.1016/S0021-9258(19)39351-2
Y Okada, H Nagase, E D Harris, A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. Purification and characterization. Journal of Biological Chemistry. ,vol. 261, pp. 14245- 14255 ,(1986) , 10.1016/S0021-9258(18)67011-5
Tomohiko Onodera, Tamotsu Toba, Tadashi Shimamura, Saburo Horiuchi, Effects of the existence of nitric oxide and the degradation of extracellular matrix on CD44 mRNA expression in cultured chondrocytes Journal of Bone and Mineral Metabolism. ,vol. 16, pp. 178- 185 ,(1998) , 10.1007/S007740050043
Srinivasan Chandrasekhar, Anita K. Harvey, Jill D. Higginbotham, Walter E. Horton, Interleukin-1-induced suppression of type II collagen gene transcription involves DNA regulatory elements. Experimental Cell Research. ,vol. 191, pp. 105- 114 ,(1990) , 10.1016/0014-4827(90)90042-9
Y. Kato, M. Iwamoto, T. Koike, F. Suzuki, Y. Takano, Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes: regulation by transforming growth factor beta and serum factors. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 9552- 9556 ,(1988) , 10.1073/PNAS.85.24.9552
Endy Chung, R.Kent Rhodes, Edward J. Miller, Isolation of three collagenous components of probable basement membrane origin from several tissues Biochemical and Biophysical Research Communications. ,vol. 71, pp. 1167- 1174 ,(1976) , 10.1016/0006-291X(76)90776-2
Kazushi Aibe, Junnosuke Ryu, Seiji Sano, Effects of hyaluronic acid on cartilage metabolism in free chondrocytes Journal of Orthopaedic Science. ,vol. 1, pp. 268- 276 ,(1996) , 10.1007/BF02348786
J. M. Dayer, S. M. Krane, R. G. Russell, D. R. Robinson, Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 945- 949 ,(1976) , 10.1073/PNAS.73.3.945
William P. Arend, Jean-Michel Dayer, Inhibition of the production and effects of interleukins‐1 and tumor necrosis factor α in rheumatoid arthritis Arthritis & Rheumatism. ,vol. 38, pp. 151- 160 ,(1995) , 10.1002/ART.1780380202
Y. Shimomura, T. Yoneda, F. Suzuki, Osteogenesis by chondrocytes from growth cartilage of rat rib. Calcified Tissue International. ,vol. 19, pp. 179- 187 ,(1975) , 10.1007/BF02564002