Loss of N-myc function results in embryonic lethality and failure of the epithelial component of the embryo to develop.

摘要: myc genes are thought to function in the processes of cellular proliferation and differentiation. To gain insight into role N-myc gene during embryogenesis, we examined its expression embryos postimplantation development using RNA situ hybridization. Tissue- cell-specific patterns unique as compared with related c-myc were observed. transcripts become progressively restricted specific cell types, primarily epithelial tissues including those developing nervous system organs characterized by epithelio-mesenchymal interaction. In contrast, confined mesenchymal compartments. These data suggest that proteins may interact different substrates performing their embryogenesis further there linked regulatory mechanisms for normal embryo. We have mutated locus via homologous recombination embryonic stem (ES) cells introduced allele mouse germ line. Live-born heterozygotes under-represented but appear normal. Homozygous mutant die prenatally at approximately 11.5 days gestation. Histologic examination homozygous indicates several affected. include central peripheral systems, mesonephros, lung, gut. Thus, is required pathology observed consistent pattern expression. Examination existence coordinate regulation embryogenesis.